KCNH2 Variant V341G Detail

We estimate the penetrance of LQTS for KCNH2 V341G is 10%. We are unaware of any observations of this variant in individuals. V341G is not present in gnomAD. V341G has not been functionally characterized. This residue is located in a Non_Hotspot region for LQT2. In silico predictions, functional data (if available), and location in structure are equivalent to observing 1 individuals with LQT2 and 9 unaffected individuals.These data combined with observations of carriers lead us to estimate the LQTS penetrance for KCNQ1 V341G around 10% (1/10).

In Silico Data

PROVEAN PolyPhen-2 BLAST-PSSM REVEL Penetrance Density (%)
-2.729 0.006 -3 0.744 1
PROVEAN scores less than -2 are considered deleterious. REVEL scores higher than 0.5 or 0.75 are considered likely pathogenic (higher sensitivity with the former cutoff, higher specificity with the latter cutoff). A PolyPhen-2 score of 0.85 or greater is considered likely pathogenic. BLAST-PSSM reflects the evolutionary conservation of residue substitutions, more negative numbers indicate fewer observations of the specific substitution than is expected. Penetrance density is our previously published method to calculate the average LQTS probability density in a shell of residues surrounding a residue of interest (Kroncke et al. 2019).

Reported Carrier Data

PubMed ID Year Carriers Unaffected LQT2 Other Disease
LITERATURE, COHORT, AND GNOMAD: - 0 0 0 -
VARIANT FEATURES ALONE: - 10 9 1 -
Summary totals might not agree with the literature table because of duplicate patients, which were excluded from the total counts. We do not distinguish here between multiple missense codons. Missense variants are combined across degenerate codon substitutions since codon-level data were not consistently available for curation.

V341G has 31 previously observed neighbors within 15 angstroms

A residue within a folded protein on average has nearest neighbors that fall roughly into two shells: a "nearest" neighbor around 5-6 angstroms and a second shell around 11 angstroms. NOTE: some residues appear multiple times at different distances since the functional KV11.1 channel (protein product of KCNH2/hERG) is a homotetramer and occasionally the same residue from multiple subunits is present within the 15A window. All variants shown in the rightmost column have been observed in at least one individual in the literature or gnomAD.

Neighbor Distance (Angstroms) Variants Observed in Individuals
341 0
340 4 F340L, F340L, F340L,
342 4 D342X, D342A, D342V,
339 5
343 5 L343fsX,
338 7
344 7
337 8 T337X, T337S, T337S,
345 8 G345S,
336 8
346 8 D346E, D346Y, D346N, D346E,
335 9 Q335X,
347 9 P347S,
334 10 P334L,
348 10
333 11
349 11
332 11
350 11
331 12 S331N, S331T,
351 12 S351L,
330 13 I330V,
352 13
329 13
353 13 T353S, T353S,
328 14 R328C, R328H, R328fsX,
354 14
327 14 Y327H,
355 14 D355G,
326 15 R326H, R326fsX, R326C,
356 15 R356H, R356C,