KCNH2 Variant I395M

Summary of observed carriers, functional annotations, and structural context for KCNH2 I395M. Data combine curated literature, international cohorts, and gnomAD observations.

Estimated LQT2 penetrance

9%

0/10 effective observations

Total carriers

0

0 LQT2 · 0 unaffected

Functional studies

0

Publications with functional data

I395M has not been reported in gnomAD. This residue resides in a Non_Hotspot region for LQT2.

We have tested the trafficking efficiency of this variant: 128% of WT with a standard error of 23%. In our analysis we used SE < 20% as 'high quality'. Approximately below 50% of WT is considered PS3 moderate and below 30% is PS3 strong.

Variant features alone are equivalent to phenotyping 0 individuals with LQT2 and 10 unaffected individuals.

In silico predictors

Variant-level computational predictors.
PROVEAN PolyPhen-2 BLAST-PSSM REVEL Penetrance Density LQT2 (%)
-2.558 0.63 1 0.647 4

PROVEAN scores below -2 suggest deleterious impact. REVEL scores above 0.5–0.75 are often interpreted as likely pathogenic. PolyPhen-2 scores above 0.85 are typically pathogenic. BLAST-PSSM reflects evolutionary conservation; more negative values indicate rarer substitutions. Penetrance density summarises neighbouring residue risk (Kroncke et al. 2019).

Reported carrier data

Observed carriers by publication or cohort.
Source Year Carriers Unaffected LQT2 Other Disease
Literature, cohort, and gnomAD 0 0 0
Variant features alone 10 10 0

Totals may differ from individual publications due to duplicate patients removed during curation.

Nearby variants

Neighbouring residues within 15 Ångström provide structural context. Variants listed in the right-most column have been observed clinically or in gnomAD. Note that some residues appear multiple times at different distances since the functional KV11.1 channel (protein product of KCNH2/hERG) is a homotetramer and occasionally the same residue from multiple subunits is present within the 15Å window.

Previously observed variants near I395M.
Neighbour residue Distance (Å) Observed variants
395 0
394 4 R394H, R394C,
396 4
393 5 P393L,
397 5 R397X, R397H,
392 7
398 7 W398L, W398X,
391 8 Q391X,
399 8
390 8
400 8 I400N,
389 9
401 9
388 10
402 10 H402R,
387 11
403 11
386 11 P386X,
404 11
385 12
405 12
384 13
406 13
383 13
407 13
382 14 A382T,
408 14
381 14
409 14 V409L, V409M, V409L,
380 15 L380X,
410 15 W410X,