KCNH2 Variant V533L Detail

We estimate the penetrance of LQTS for KCNH2 V533L is 21%. We are unaware of any observations of this variant in individuals. V533L is not present in gnomAD. We have tested the trafficking efficiency of this variant, 47% of WT with a standard error of 19%; in our analysis we used SE < 20% as 'high quality'. Approximately below 50% of WT is considered PS3 moderate and below 30% is PS3 strong. V533L has not been functionally characterized. This residue is located in a Hotspot region for LQT2. In silico predictions, functional data (if available), and location in structure are equivalent to observing 2 individuals with LQT2 and 8 unaffected individuals.These data combined with observations of carriers lead us to estimate the LQTS penetrance for KCNQ1 V533L around 21% (2/10).

In Silico Data

PROVEAN PolyPhen-2 BLAST-PSSM REVEL Penetrance Density (%)
-2.875 0.771 1 0.839 72
PROVEAN scores less than -2 are considered deleterious. REVEL scores higher than 0.5 or 0.75 are considered likely pathogenic (higher sensitivity with the former cutoff, higher specificity with the latter cutoff). A PolyPhen-2 score of 0.85 or greater is considered likely pathogenic. BLAST-PSSM reflects the evolutionary conservation of residue substitutions, more negative numbers indicate fewer observations of the specific substitution than is expected. Penetrance density is our previously published method to calculate the average LQTS probability density in a shell of residues surrounding a residue of interest (Kroncke et al. 2019).

Reported Carrier Data

PubMed ID Year Carriers Unaffected LQT2 Other Disease
LITERATURE, COHORT, AND GNOMAD: - 0 0 0 -
VARIANT FEATURES ALONE: - 10 8 2 -
Summary totals might not agree with the literature table because of duplicate patients, which were excluded from the total counts. We do not distinguish here between multiple missense codons. Missense variants are combined across degenerate codon substitutions since codon-level data were not consistently available for curation.

V533L has 45 previously observed neighbors within 15 angstroms

A residue within a folded protein on average has nearest neighbors that fall roughly into two shells: a "nearest" neighbor around 5-6 angstroms and a second shell around 11 angstroms. NOTE: some residues appear multiple times at different distances since the functional KV11.1 channel (protein product of KCNH2/hERG) is a homotetramer and occasionally the same residue from multiple subunits is present within the 15A window. All variants shown in the rightmost column have been observed in at least one individual in the literature or gnomAD.

Neighbor Distance (Angstroms) Variants Observed in Individuals
533 0
532 5
534 5 R534C,
536 6 A536X,
535 6 V535M,
530 6
537 6 R537W,
500 7 I500Del,
497 7 W497L, W497X,
501 7 D501Y, D501H, D501N,
531 8 R531Del, R531W, R531Q,
538 8
529 9
418 9
504 10 A504V,
539 10
527 11
503 11
463 11 F463L, F463L, F463L,
414 11 I414fsX,
499 11
496 11
415 11
502 11 M502I, M502I, M502I,
421 12 T421M, T421fsX,
498 12
422 12 A422T,
542 12
466 12 D466E, D466E,
419 13
411 13
528 13 R528P, R528X, R528W,
417 13
493 13 Y493Ins, Y493C, Y493F, Y493H,
505 13 A505V,
470 13 N470D,
467 13
540 14 D540fsX,
526 14
552 14 L552S,
556 14
563 14 W563X, W563C, W563C, W563G,
559 14 L559H, L559F,
541 15 R541C, R541H,
425 15