SCN5A Variant G1519R Detail

We estimate the penetrance of LQTS for SCN5A G1519R around 19% and the Brugada syndrome penetrance around 21%. SCN5A G1519R was found in a total of 0 carriers in 0 papers and/or in gnomAD: 0 had Brugada syndrome, 0 had LQTS. G1519R is not present in gnomAD. G1519R has been functionally characterized in 0 papers. This residue is located in a Mild_Hotspot region for Brugada syndrome and a Mild_Hotspot region for LQTS. In silico predictions, functional data (if available), and location in structure are equivalent to phenotyping 10 individuals for Brugada syndrome (2 diagnosed with Brugada syndrome) and 5 individuals for LQTS (0 with LQTS). These data combined with observations of carriers lead us to estimate the LQTS penetrance for SCN5A G1519R around 19% (0/10) and the Brugada syndrome penetrance around 21% (2/10).

In Silico Data

PROVEAN PolyPhen-2 BLAST-PSSM REVEL Penetrance Density BrS (%) Penetrance Density LQT3 (%)
NA NA NA 0.915 24 22
PROVEAN scores less than -2 are considered deleterious. REVEL scores higher than 0.5 or 0.75 are considered likely pathogenic (higher sensitivity with the former cutoff, higher specificity with the latter cutoff). A PolyPhen-2 score of 0.85 or greater is considered likely pathogenic. BLAST-PSSM reflects the evolutionary conservation of residue substitutions, more negative numbers indicate fewer observations of the specific substitution than is expected. Penetrance Density is our previously published method to calculate the average BrS/LQTS probability density in a shell of residues surrounding a residue of interest (Kroncke et al. 2019).

Reported Carrier Data

PubMed ID Year Carriers Unaffected LQT3 BrS1 Other Other Disease
LITERATURE, COHORT, AND GNOMAD: - 0 0 0 0 -
VARIANT FEATURES ALONE: - 15 13 0 2 - -
Summary totals might not agree with the literature table because of duplicate patients, which were excluded from the total counts. We do not distinguish here between multiple missense codons. Missense variants are combined across degenerate codon substitutions since codon-level data were not consistently available for curation.

G1519R has 29 previously observed neighbors within 15 angstroms

A residue within a folded protein on average has nearest neighbors that fall roughly into two shells: a "nearest" neighbor around 5-6 angstroms and a second shell around 11 angstroms. All variants shown in the rightmost column have been observed in at least one individual in the literature or gnomAD.

Neighbor Distance (Angstroms) Variants Observed in Individuals
1523 5 D1523N,
1521 5 I1521K, I1521T,
1585 15 Y1585C,
1527 12 K1527R,
1576 13
1517 7
1525 10 V1525M, V1525A,
1519 0
1515 7 N1515S, c.4542+15G>A,
1524 8 I1524T,
1512 6 R1512W, R1512L, R1512Q,
1530 13
1514 8 L1514M,
1518 5
1509 15 P1509T,
1578 13 c.4732_4733dupAA,
1526 10 T1526P,
1516 9 L1516sp,
1583 12 R1583C, R1583H,
1580 9
1511 11
1513 6
1581 8 A1581S,
1582 11 L1582P,
1579 13 L1579fsX53,
1522 6
1520 4
1577 10
1510 12