SCN5A Variant A1580P Detail

We estimate the penetrance of LQTS for SCN5A A1580P around 3% and the Brugada syndrome penetrance around 39%. SCN5A A1580P was found in a total of 0 carriers in 0 papers and/or in gnomAD: 0 had Brugada syndrome, 0 had LQTS. A1580P is not present in gnomAD. A1580P has been functionally characterized in 0 papers. This residue is located in a Hotspot region for Brugada syndrome and a Non_Hotspot region for LQTS. In silico predictions, functional data (if available), and location in structure are equivalent to phenotyping 10 individuals for Brugada syndrome (3 diagnosed with Brugada syndrome) and 5 individuals for LQTS (0 with LQTS). These data combined with observations of carriers lead us to estimate the LQTS penetrance for SCN5A A1580P around 3% (0/10) and the Brugada syndrome penetrance around 39% (3/10).

In Silico Data

PROVEAN PolyPhen-2 BLAST-PSSM REVEL Penetrance Density BrS (%) Penetrance Density LQT3 (%)
NA NA NA 0.667 58 0
PROVEAN scores less than -2 are considered deleterious. REVEL scores higher than 0.5 or 0.75 are considered likely pathogenic (higher sensitivity with the former cutoff, higher specificity with the latter cutoff). A PolyPhen-2 score of 0.85 or greater is considered likely pathogenic. BLAST-PSSM reflects the evolutionary conservation of residue substitutions, more negative numbers indicate fewer observations of the specific substitution than is expected. Penetrance Density is our previously published method to calculate the average BrS/LQTS probability density in a shell of residues surrounding a residue of interest (Kroncke et al. 2019).

Reported Carrier Data

PubMed ID Year Carriers Unaffected LQT3 BrS1 Other Other Disease
LITERATURE, COHORT, AND GNOMAD: - 0 0 0 0 -
VARIANT FEATURES ALONE: - 15 12 0 3 - -
Summary totals might not agree with the literature table because of duplicate patients, which were excluded from the total counts. We do not distinguish here between multiple missense codons. Missense variants are combined across degenerate codon substitutions since codon-level data were not consistently available for curation.

A1580P has 41 previously observed neighbors within 15 angstroms

A residue within a folded protein on average has nearest neighbors that fall roughly into two shells: a "nearest" neighbor around 5-6 angstroms and a second shell around 11 angstroms. All variants shown in the rightmost column have been observed in at least one individual in the literature or gnomAD.

Neighbor Distance (Angstroms) Variants Observed in Individuals
1523 11 D1523N,
1521 6 I1521T, I1521K,
1585 11 Y1585C,
1527 15 K1527R,
1576 7
1517 12
1525 9 V1525M, V1525A,
1519 9
1572 12
1596 13 F1596C, F1596I,
1589 14
1584 10
1515 12 c.4542+15G>A, N1515S,
1574 11 c.4719C>T, E1574K,
1524 10 I1524T,
1512 11 R1512W, R1512L, R1512Q,
1530 13
1586 9
1514 12 L1514M,
1518 6
1592 12
1578 8 c.4732_4733dupAA,
1573 10
1587 11 F1587V,
1526 12 T1526P,
1516 13 L1516sp,
1583 6 R1583C, R1583H,
1580 0
1588 14 T1588I,
1511 12
1581 4 A1581S,
1582 6 L1582P,
1579 4 L1579fsX53,
1513 8
1522 8
1593 15 I1593M,
1520 11
1577 6
1595 14
1575 9 C1575S,
1510 11