KCNH2 Variant A448T

Summary of observed carriers, functional annotations, and structural context for KCNH2 A448T. Data combine curated literature, international cohorts, and gnomAD observations.

Estimated LQT2 penetrance

12%

2/17 effective observations

Total carriers

7

1 LQT2 · 0 unaffected

Functional studies

0

Publications with functional data

A448T is present in 6 alleles in gnomAD. This residue resides in a Hotspot region for LQT2.

We have tested the trafficking efficiency of this variant: 112% of WT with a standard error of 26%. In our analysis we used SE < 20% as 'high quality'. Approximately below 50% of WT is considered PS3 moderate and below 30% is PS3 strong.

Variant features alone are equivalent to phenotyping 1 individuals with LQT2 and 9 unaffected individuals.

In silico predictors

Variant-level computational predictors.
PROVEAN PolyPhen-2 BLAST-PSSM REVEL Penetrance Density LQT2 (%)
0.439 0.121 1 0.514 48

PROVEAN scores below -2 suggest deleterious impact. REVEL scores above 0.5–0.75 are often interpreted as likely pathogenic. PolyPhen-2 scores above 0.85 are typically pathogenic. BLAST-PSSM reflects evolutionary conservation; more negative values indicate rarer substitutions. Penetrance density summarises neighbouring residue risk (Kroncke et al. 2019).

Reported carrier data

Observed carriers by publication or cohort.
Source Year Carriers Unaffected LQT2 Other Disease
22727609 2012 1 0 1
Literature, cohort, and gnomAD 7 0 1
Variant features alone 10 9 1

Totals may differ from individual publications due to duplicate patients removed during curation.

Nearby variants

Neighbouring residues within 15 Ångström provide structural context. Variants listed in the right-most column have been observed clinically or in gnomAD. Note that some residues appear multiple times at different distances since the functional KV11.1 channel (protein product of KCNH2/hERG) is a homotetramer and occasionally the same residue from multiple subunits is present within the 15Å window.

Previously observed variants near A448T.
Neighbour residue Distance (Å) Observed variants
448 0 A448T, A448S,
447 4 Y447X,
449 4
446 5
450 5
445 7
451 7 P451L,
444 8 E444K, E444D, E444D,
452 8
443 8 T443fsX, T443N,
453 8
442 9
454 9
441 10 P441R, P441L,
455 10
440 11 P440L,
456 11 D456Y,
439 11
457 11 L457P,
438 12 E438K, E438X,
458 12
437 13
459 13
436 13 T436M,
460 13 D460fsX,
435 14 E435G, E435X,
461 14
434 14
462 14 M462Ins,
433 15
463 15 F463L, F463L, F463L