KCNH2 Variant N635I

Summary of observed carriers, functional annotations, and structural context for KCNH2 N635I. Data combine curated literature, international cohorts, and gnomAD observations.

Estimated LQT2 penetrance

39%

90% CI: 17.3% – 63.8%

4/11 effective observations

Total carriers

1

1 LQT2 · 0 unaffected

Functional studies

0

Publications with functional data

N635I has not been reported in gnomAD. This residue resides in a Hotspot region for LQT2.

We have tested the trafficking efficiency of this variant: 20% of WT with a standard error of 11%. In our analysis we used SE < 20% as 'high quality'. Approximately below 50% of WT is considered PS3 moderate and below 30% is PS3 strong.

Variant features alone are equivalent to phenotyping 3 individuals with LQT2 and 7 unaffected individuals.

In silico predictors

Variant-level computational predictors.
PROVEAN PolyPhen-2 BLAST-PSSM REVEL Penetrance Density LQT2 (%)
-8.534 0.786 -4 0.913 91

PROVEAN scores below -2 suggest deleterious impact. REVEL scores above 0.5–0.75 are often interpreted as likely pathogenic. PolyPhen-2 scores above 0.85 are typically pathogenic. BLAST-PSSM reflects evolutionary conservation; more negative values indicate rarer substitutions. Penetrance density summarises neighbouring residue risk (Kroncke et al. 2019).

Reported carrier data

Observed carriers by publication or cohort.
Source Year Carriers Unaffected LQT2 Other Disease
15840476 2005 1 0 1
Literature, cohort, and gnomAD 1 0 1
Variant features alone 10 7 3

Totals may differ from individual publications due to duplicate patients removed during curation.

Nearby variants

Neighbouring residues within 15 Ångström provide structural context. Variants listed in the right-most column have been observed clinically or in gnomAD. Note that some residues appear multiple times at different distances since the functional KV11.1 channel (protein product of KCNH2/hERG) is a homotetramer and occasionally the same residue from multiple subunits is present within the 15Å window.

Previously observed variants near N635I.
Neighbour residue Distance (Å) Observed variants
635 0 N635I,
634 4 T634P, T634A, T634S, T634S, T634I,
636 5
638 5 K638E, K638D, K638Del, K638R,
609 6 D609N, D609G,
612 6 V612L, V612L, V612A,
608 6
639 7 I639F, I639N,
637 7 E637K, E637G, E637X,
633 8 N633D, N633S, N633I,
613 8 T613A, T613L, T613K, T613M,
606 9 S606Del, S606P, S606F,
632 9 P632A, P632S,
593 9 I593V, I593K, I593T, I593R, I593X,
616 10 Y616S,
575 10 E575K,
611 10 Y611D,
610 10
571 11 I571L, I571V,
607 11
584 11 G584S, G584R, G584C,
641 11 S641P, S641F,
589 11 L589P,
642 11 I642V, I642Del,
640 11 F640L, F640V, F640Del, F640L, F640L,
614 11 A614T, A614V,
583 11 I583V,
615 11 L615V, L615F,
605 11 P605L,
592 12 Q592X,
595 12 K595E, K595N, K595N,
629 12 N629D, N629T, N629S, N629I, N629K, N629K,
594 12
631 12 S631F,
572 12 G572S, G572R, G572C, G572D,
568 13 W568C, W568C,
585 13 W585C, W585C,
630 13 V630I, V630T, V630A,
590 13 G590D, G590V,
643 13
577 13
604 14 G604S, G604C, G604D,
627 14 F627L, F627fsX, F627X, F627L, F627L,
617 14 F617L, F617V, F617L, F617L,
570 14
586 15 L586M,
585 15 W585C, W585C,
576 15
574 15 M574L, M574V, M574L,
619 15
565 15
645 15 M645L, M645V, M645L, M645R, M645I, M645I, M645I