SCN5A Variant P336L

Summary of observed carriers, functional annotations, and structural context for SCN5A P336L. Data combine curated literature, international cohorts, and gnomAD observations.

Estimated LQT3 penetrance

12%

0/13 effective observations

Estimated BrS1 penetrance

38%

4/13 effective observations

Total carriers

3

1 BrS1 · 0 LQT3 · 2 unaffected

P336L has not been reported in gnomAD. This residue resides in a Hotspot region for Brugada syndrome and a Mild_Hotspot region for LQT3.

Variant features alone are equivalent to phenotyping 3 individuals for Brugada syndrome and 0 individuals for LQT3.

In silico predictors

Variant-level computational predictors.
PROVEAN PolyPhen-2 BLAST-PSSM REVEL Penetrance Density BrS (%) Penetrance Density LQT3 (%)
-9.68 0.998 -2.24 0.916 50 15

PROVEAN scores below -2 suggest deleterious impact. REVEL scores above 0.5–0.75 are often interpreted as likely pathogenic. PolyPhen-2 scores above 0.85 are typically pathogenic. Penetrance density summarises neighbouring residue risk (Kroncke et al. 2019).

Reported carrier data

Observed carriers by publication or cohort.
Source Year Carriers Unaffected LQT3 BrS1 Other Other Disease
17075016 2006 3 0 1 0
24269159 2013 2 0 1 0
26173111 2015 1 0 1 0
20129283 2010 1 0 1 0
20129283 2010 1 0 1 0
30059973 2018 1 1 0 0
Literature, cohort, and gnomAD 3 2 0 1
Variant features alone 15 12 0 3

Totals may differ from individual publications due to duplicate patients removed during curation.

Functional data

Peak and late/persistent current values are relative to wild-type (100% indicates no change). V1/2 activation and inactivation denote the membrane potentials (mV) at which half-maximal current is achieved.

Published electrophysiology measurements.
PubMed ID Year Cell Type Peak Current (% WT) V1/2 Activation (mV) V1/2 Inactivation (mV) Late/Persistent Current (% WT)
17075016 2006 HEK-tSA201 15 1.7 -1.5 0
24269159 2013 HEK 20 0
26173111 2015
20129283 2010
20129283 2010
30059973 2018

Nearby variants

Neighbouring residues within 15 Ångström provide structural context. Variants listed in the right-most column have been observed clinically or in gnomAD.

Previously observed variants near P336L.
Neighbour residue Distance (Å) Observed variants
328 12
333 10 c.998+1G>A, c.998+5G>A,
1689 13 D1689N,
341 10 C341Y,
342 12
326 7
335 4 C335S, C335R, C335S,
327 10
339 4
384 14 S384T,
325 12 L325R,
1684 14 W1684R, W1684R,
284 13
336 0 P336L,
1690 12 c.5068_5070delGA, D1690N
329 14
282 9 R282C, R282H,
279 12
324 11
340 10 R340W, R340Q,
338 6
285 15 T285K,
278 14 H278D, H278R,
1691 14
334 7 c.999-424_1338+81del,
383 11
280 7 C280Y,
281 10 V281M,
323 12
283 11
337 6
332 12 A332T,