SCN5A Variant A332T

Summary of observed carriers, functional annotations, and structural context for SCN5A A332T. Data combine curated literature, international cohorts, and gnomAD observations.

Estimated LQT3 penetrance

27%

2/13 effective observations

Estimated BrS1 penetrance

15%

1/13 effective observations

Total carriers

3

0 BrS1 · 1 LQT3 · 2 unaffected

A332T is present in 2 alleles in gnomAD. This residue resides in a Mild_Hotspot region for Brugada syndrome and a Mild_Hotspot region for LQT3.

Variant features alone are equivalent to phenotyping 1 individuals for Brugada syndrome and 1 individuals for LQT3.

In silico predictors

Variant-level computational predictors.
PROVEAN PolyPhen-2 BLAST-PSSM REVEL Penetrance Density BrS (%) Penetrance Density LQT3 (%)
-3.13 0.271 -0.15 0.681 25 30

PROVEAN scores below -2 suggest deleterious impact. REVEL scores above 0.5–0.75 are often interpreted as likely pathogenic. PolyPhen-2 scores above 0.85 are typically pathogenic. Penetrance density summarises neighbouring residue risk (Kroncke et al. 2019).

Reported carrier data

Observed carriers by publication or cohort.
Source Year Carriers Unaffected LQT3 BrS1 Other Other Disease
30059973 2018 1 1 0 0
Literature, cohort, and gnomAD 3 2 1 0
Variant features alone 15 13 1 1

Totals may differ from individual publications due to duplicate patients removed during curation.

Functional data

Peak and late/persistent current values are relative to wild-type (100% indicates no change). V1/2 activation and inactivation denote the membrane potentials (mV) at which half-maximal current is achieved.

Published electrophysiology measurements.
PubMed ID Year Cell Type Peak Current (% WT) V1/2 Activation (mV) V1/2 Inactivation (mV) Late/Persistent Current (% WT)
30059973 2018

Nearby variants

Neighbouring residues within 15 Ångström provide structural context. Variants listed in the right-most column have been observed clinically or in gnomAD.

Previously observed variants near A332T.
Neighbour residue Distance (Å) Observed variants
328 7
333 3 c.998+1G>A, c.998+5G>A,
1702 11
326 7
276 13 L276Q, L276P,
387 7
279 14
385 6 A385T,
330 6 S330F,
278 11 H278D, H278R,
388 9 I388S,
1698 12 A1698T
334 5 c.999-424_1338+81del,
332 0 A332T,
327 6
1695 15 Q1695X,
384 6 S384T,
1688 15
329 4
1692 10
386 5 G386R, G386R, G386E,
1693 12
378 12
1699 11
331 5
379 11
1703 15
272 14
341 11 C341Y,
335 9 C335S, C335R, C335S,
325 9 L325R,
1690 11 c.5068_5070delGA, D1690N,
324 12
1697 15
389 10 Y389H, Y389X,
393 15
390 13
275 12 N275K, N275K,
383 6
280 12 C280Y,
323 15
382 7
1696 13
1689 10 D1689N,
342 15
336 12 P336L,
381 9 c.1140+1G>A, c.1141-3C>A,
1691 7
380 12