SCN5A Variant E312K

Summary of observed carriers, functional annotations, and structural context for SCN5A E312K. Data combine curated literature, international cohorts, and gnomAD observations.

Estimated LQT3 penetrance

1%

0/11 effective observations

Estimated BrS1 penetrance

57%

6/11 effective observations

Total carriers

1

1 BrS1 · 0 LQT3 · 0 unaffected

E312K has not been reported in gnomAD. This residue resides in a Hotspot region for Brugada syndrome and a Non_Hotspot region for LQT3.

Variant features alone are equivalent to phenotyping 5 individuals for Brugada syndrome and 0 individuals for LQT3.

In silico predictors

Variant-level computational predictors.
PROVEAN PolyPhen-2 BLAST-PSSM REVEL Penetrance Density BrS (%) Penetrance Density LQT3 (%)
-1.06 0.024 -1.19 0.381 88 0

PROVEAN scores below -2 suggest deleterious impact. REVEL scores above 0.5–0.75 are often interpreted as likely pathogenic. PolyPhen-2 scores above 0.85 are typically pathogenic. Penetrance density summarises neighbouring residue risk (Kroncke et al. 2019).

Reported carrier data

Observed carriers by publication or cohort.
Source Year Carriers Unaffected LQT3 BrS1 Other Other Disease
25650408 2015 1 0 1 0
Literature, cohort, and gnomAD 1 0 0 1
Variant features alone 15 10 0 5

Totals may differ from individual publications due to duplicate patients removed during curation.

Functional data

Peak and late/persistent current values are relative to wild-type (100% indicates no change). V1/2 activation and inactivation denote the membrane potentials (mV) at which half-maximal current is achieved.

Published electrophysiology measurements.
PubMed ID Year Cell Type Peak Current (% WT) V1/2 Activation (mV) V1/2 Inactivation (mV) Late/Persistent Current (% WT)
25650408 2015

Nearby variants

Neighbouring residues within 15 Ångström provide structural context. Variants listed in the right-most column have been observed clinically or in gnomAD.

Previously observed variants near E312K.
Neighbour residue Distance (Å) Observed variants
297 15
298 14 G298S, G298D,
299 14 L299F, L299M, L299F,
300 13 V300I,
301 13
302 12
303 11
304 11
305 10 D305N
306 9 L306F, L306V,
307 8
308 8
309 7 S309C, S309N,
310 5 D310N,
311 4
312 0 E312K, c.934+1G>A, c.934+4C>T,
313 4
314 5
315 7 L315P,
316 8
317 8 K317N, K317E, K317N, K317M,
318 9
319 10 G319S, G319C, G319R,
320 11 T320N,
321 11 S321Y,
322 12
323 13
324 13
325 14 L325R,
326 14
327 15