SCN5A Variant c.3963+2T>C

Summary of observed carriers, functional annotations, and structural context for SCN5A c.3963+2T>C. Data combine curated literature, international cohorts, and gnomAD observations.

Estimated LQT3 penetrance

4%

0/13 effective observations

Estimated BrS1 penetrance

50%

6/13 effective observations

Total carriers

3

2 BrS1 · 0 LQT3 · 1 unaffected

c.3963+2T>C has not been reported in gnomAD. This residue resides in a Hotspot region for Brugada syndrome and a Non_Hotspot region for LQT3.

Variant features alone are equivalent to phenotyping 4 individuals for Brugada syndrome and 0 individuals for LQT3.

In silico predictors

Variant-level computational predictors.
PROVEAN PolyPhen-2 BLAST-PSSM REVEL Penetrance Density BrS (%) Penetrance Density LQT3 (%)
NA NA NA None 66 5

PROVEAN scores below -2 suggest deleterious impact. REVEL scores above 0.5–0.75 are often interpreted as likely pathogenic. PolyPhen-2 scores above 0.85 are typically pathogenic. Penetrance density summarises neighbouring residue risk (Kroncke et al. 2019).

Reported carrier data

Observed carriers by publication or cohort.
Source Year Carriers Unaffected LQT3 BrS1 Other Other Disease
10471492 1999 15 0 0 15 PCCD
16643399 2006 1 0 1 0
20031634 2009 9 0 2 6 PCCD
22717692 2012 20 0 0 20 Conduction defects
20129283 2010 1 0 1 0
Literature, cohort, and gnomAD 3 1 0 2
Variant features alone 15 11 0 4

Totals may differ from individual publications due to duplicate patients removed during curation.

Functional data

Peak and late/persistent current values are relative to wild-type (100% indicates no change). V1/2 activation and inactivation denote the membrane potentials (mV) at which half-maximal current is achieved.

Published electrophysiology measurements.
PubMed ID Year Cell Type Peak Current (% WT) V1/2 Activation (mV) V1/2 Inactivation (mV) Late/Persistent Current (% WT)
10471492 1999
16643399 2006
20031634 2009
22717692 2012
20129283 2010

Nearby variants

Neighbouring residues within 15 Ångström provide structural context. Variants listed in the right-most column have been observed clinically or in gnomAD.

Previously observed variants near c.3963+2T>C.
Neighbour residue Distance (Å) Observed variants
1328 10 V1328M,
1659 9
1480 7 c.4437+5G>A, c.4438-1C>T,
1773 12
1653 12
1472 11 p.N1472del, N1472S,
1315 9
1771 14 I1771T,
1652 14 M1652T, M1652R,
1314 11 c.3940_3941delCT,
1320 6 M1320I, M1320I, M1320I,
1656 8
1477 7 K1477N, K1477N,
1471 14
1762 14 p.I1762del, I1762M,
1470 13
1478 12 K1478E,
1767 13 Y1767C,
1313 14
1660 10 I1660V, I1660S,
1654 14
1329 11 G1329S,
1769 11
1316 13 R1316Q, R1316L,
1766 10 M1766L, M1766V, M1766L, M1766T,
1319 5 G1319V,
1768 15 I1768V,
1774 12 N1774D, c.5321_5324dupACTT
1479 9
1473 7 F1473S, F1473C,
1663 12
1657 11
1474 11
1662 13
1324 6
1481 11 G1481R, G1481R, G1481E, G1481V,
1317 10 F1317C,
1327 9
1318 8
1330 12 A1330T, A1330P, A1330D,
1321 7 R1321K,
1323 4 V1323G,
1770 9 I1770V,
1482 13
1322 0 c.3963+2T>C, c.3963+4A>G,
1312 13
1326 7 A1326S,
1763 13 V1763M, V1763L, V1763L,
1311 15 L1311P,
1476 5 Q1476X, Q1476R,
1661 13 G1661R, G1661R, G1661E,
1331 15 I1331V,
1655 11
1475 11 p.Q1475NfsX6, Q1475L,
1469 12 I1469V,
1325 6 N1325S,
1658 13