SCN5A Variant V1328M

Summary of observed carriers, functional annotations, and structural context for SCN5A V1328M. Data combine curated literature, international cohorts, and gnomAD observations.

Estimated LQT3 penetrance

24%

1/11 effective observations

Estimated BrS1 penetrance

37%

4/11 effective observations

Total carriers

1

1 BrS1 · 0 LQT3 · 0 unaffected

V1328M has not been reported in gnomAD. This residue resides in a Hotspot region for Brugada syndrome and a Mild_Hotspot region for LQT3.

Variant features alone are equivalent to phenotyping 3 individuals for Brugada syndrome and 1 individuals for LQT3.

In silico predictors

Variant-level computational predictors.
PROVEAN PolyPhen-2 BLAST-PSSM REVEL Penetrance Density BrS (%) Penetrance Density LQT3 (%)
-1.96 0.988 3.37 0.775 50 35

PROVEAN scores below -2 suggest deleterious impact. REVEL scores above 0.5–0.75 are often interpreted as likely pathogenic. PolyPhen-2 scores above 0.85 are typically pathogenic. Penetrance density summarises neighbouring residue risk (Kroncke et al. 2019).

Reported carrier data

Observed carriers by publication or cohort.
Source Year Carriers Unaffected LQT3 BrS1 Other Other Disease
27560382 2016 1 0 1 0 drug induced BrS
28341781 2017 1 0 1 0
29574140 2018 1 0 1 0
Literature, cohort, and gnomAD 1 0 0 1
Variant features alone 15 11 1 3

Totals may differ from individual publications due to duplicate patients removed during curation.

Functional data

Peak and late/persistent current values are relative to wild-type (100% indicates no change). V1/2 activation and inactivation denote the membrane potentials (mV) at which half-maximal current is achieved.

Published electrophysiology measurements.
PubMed ID Year Cell Type Peak Current (% WT) V1/2 Activation (mV) V1/2 Inactivation (mV) Late/Persistent Current (% WT)
27560382 2016 HEK 97 2.4 7.1 50
28341781 2017
29574140 2018

Nearby variants

Neighbouring residues within 15 Ångström provide structural context. Variants listed in the right-most column have been observed clinically or in gnomAD.

Previously observed variants near V1328M.
Neighbour residue Distance (Å) Observed variants
1328 0 V1328M,
1271 10 W1271C, W1271C,
1757 14
1472 10 p.N1472del, N1472S,
1315 11
1274 15
1314 13 c.3940_3941delCT,
1320 11 M1320I, M1320I, M1320I,
1333 10
1270 11 A1270S,
1471 15
1762 11 p.I1762del, I1762M,
1660 14 I1660V, I1660S,
1329 4 G1329S,
1766 11 M1766L, M1766V, M1766L, M1766T
1319 14 G1319V,
1479 13
1473 11 F1473S, F1473C,
1334 10 I1334V,
1468 13 V1468F, V1468A,
1663 12
1324 6
1327 4
1758 12 I1758V, p.I1758del,
1330 6 A1330T, A1330P, A1330D,
1321 12 R1321K,
1323 9 V1323G,
1322 10 c.3963+2T>C, c.3963+4A>G,
1312 12
1326 6 A1326S,
1763 12 V1763M, V1763L, V1763L,
1311 12 L1311P,
1332 8 P1332Q, P1332L,
1465 15 p.F1465_L1480dup,
1476 10 Q1476X, Q1476R,
1331 6 I1331V,
1475 13 p.Q1475NfsX6, Q1475L,
1469 11 I1469V,
1336 14
1269 13 N1269S,
1325 6 N1325S,
1335 11 M1335R,
1667 15 V1667I,